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Isolation, Purification and Physico-chemical Properties of Acidic Polysaccharides from Green Tea

DING Jing-si, NIE Shao-ping*, FENG Yan-ling, WANG Jun-qiao, QUE Zhi-qiang   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Online:2014-12-15 Published:2014-12-15

Abstract:

Tea polysaccharides (TPS) were extracted and purified from green tea from Wuyuan of Jiangxi province. The
purity and molecular weight (Mw) were determined by high performance gel permeation chromatography (HPGPC). The
contents of sugar and protein, monosaccharide compositions, and uronic acid composition were determined as well. UV
and IR spectroscopy were used for determining their spectroscopic properties. The results showed that the composition was
highly homogeneous acidic polysaccharides. The Mw was 289 734 D, the content of polysaccharide was 55.1%, and the
content of protein was 1.8%. This polysaccharide was mainly composed of rhamnose, ribose, arabinose, mannose, glucose
and galactose with a molar ratio of 1.26:3.18:4.08:1.00:1.52:3.92. The major uronic acid was galacturonic acid with a
relative content of 33.5%.

Key words: tea, acidic polysaccharide, physico-chemical properties, monosaccharide composition, molecular weight

CLC Number: