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Detection of Cry2A Protein Based on Quartz Crystal Microbalance Technique

DU Fang1, HUANG Xin2, JI Shu-juan1,*   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China;
    2. Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, China
  • Online:2014-12-25 Published:2014-12-29
  • Contact: JI Shu-juan

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Abstract:

Purpose: To establish a detection method for Cry2A protein by quartz crystal microbalance (QCM) sensing
method. Methods: By using QCM, based on the principle of the interaction between antigen and antibody, the specific
monoclonal antibody was modified on the gold surface for Cry2A protein detection. Results: The proposed method had a
high sensitivity of 1 μg/mL, good specificity, and sufficient repeatability. Conclusion: This method may provide a new idea
for the detection of Bacillus thuringiensis protein from genetically modified crops and thus has promising prospects for
application in import and export inspection and quarantine supervision work.

Key words: quartz crystal microbalance (QCM), Cry2A protein, genetically modified crop (GMC)

CLC Number: