Abstract:

Crude polysaccharide was obtained from Sipunculus nudus by alkali extraction and deproteinized by Sevag
method. The polysaccharide was sequentially purified by ion exchange chromatography on DEAE-52 cellulose column
and Sephadex G-100 Gel column. Then, the purified polysaccharide from Sipunculus nudus (SNPS) was characterized
for physicochemical properties and monosaccharide composition. The results showed that SNPS with a purity of 99.35%
contained 22.21% alduronic acid without protein, nuclein or sulfate ions. Gas chromatography analysis showed that the
monosaccharide compositions of SNPS were arabinose, glucose and galactose, with a molar ratio of 1.16:9.54:1. This study
demonstrated that SNPS was a homogeneous component obtained from Sipunculus nudus.

Key words: Sipunculus nudus, polysaccharide, cellulose, gel, monosacharide