Abstract:

A highly sensitive and selective dual color fluorescence quantitative detection method for mercury (Hg2+) in rice
was developed by synchronous scanning fluorescence spectrometry. In this method, two complementary single-stranded
nucleic acids with T-T mismatches, SYBR GreenⅠ and graphene oxide were employed. Under the optimum conditions, the
total fluorescence intensity of SYBR GreenⅠ and carboxy-X-rhodamine (ROX) exhibited good linear dependence on the
quantity of Hg2+ in the range of 5 × 10-10–500 × 10-10 mol/L. The fitted regression equation was ΔIT = 2.121 3 C + 10.536 0
with a detection limit of 2 × 10-10 mol/L (3σ). The proposed method displayed a good precision, high selection, simple
operation, fast detection, low detection limit and high sensitivity. This method has been applied with satisfactory results.

Key words: mercury, quantitative detection, dual color fluorescence, rice