Abstract:

Seven fractions from the crude ethanol extract of Ya’an Tibetan tea were separated by organic solvents. The
seven fractions and the water extract of Ya’an Tibetan tea were analyzed by ELISA using a 96-well microplate reader for
lipase-inhibitory activities. The results showed that all the ethanol extract fractions (0.011–0.216 mg/mL) and the water
extract (0.018–0.360 mg/mL) possessed lipase-inhibitory activities with a significant concentration-effect relationship.
The maximum inhibitory rate of the water extract against lipase was 37.14%, while those of catechin (C), theaflavin (TF),
theabromine (TB) and thearubiginsⅡ (TRsⅡ) fractions extracted from Ya’an Tibetan tea were 44.67%, 39.46%, 30.31% and
29.53%, respectively. Lipase was inhibited in decreasing order by catechin, theaflavin and thearubigins from Ya’an Tibetan
tea, as indicated by analysis of the major components and regression analysis.

Key words: Ya’an Tibetan tea, active fraction, high-throughput screening, lipase activity, inhibition