Abstract:

A simple and rapid method was developed for determining 1,2,3,5,6-pentathiepane in Lentinula edodes by gas
chromatography-mass spectrometry (GC-MS). Samples were extracted by acetonitrile, purified by solid-phase florisil column
and detected by GC-MS under selective ion monitoring mode. Good linear correlation was obtained within the concentration
range of 1,2,3,5,6-pentathiepane between 1.0 and 20.0 μg/mL, under the chromatographic conditions used in this study.
The average recovery rates of 1,2,3,5,6-pentathiepane at spiked concentrations of 10.0, 20.0 and 30.0 mg/kg were between
86.61% and 98.48%, with relative standard deviation of 2.92%?4.28%. The detection limit and quantification limit were 0.10
and 0.25 mg/kg, respectively. This method has the advantages of convenient operation and good reproducibility, which is
suitable for determining 1,2,3,5,6-pentathiepane in Lentinula edodes.

Key words: solid-phase extraction, gas chromatography-mass spectrometry (GC-MS), 1,2,3,5,6-pentathiepane