Abstract:

In this work, we obtained antibacterial peptides by enzymatic hydrolysis of water-soluble proteins from the
residue left after supercritical carbon dioxide extraction of seed oil from sea buckthorn seeds with ProteAX protease and
pepsin respectively and purification of the resultant hydrolysates by membrane filtration. in vitro test results indicated that
the ProteAX hydrolysate possessed bacteriostasis on Staphylococcus aureus, Salmonella, and Pseudomonas aeruginosa,
while the pepsin hydrolysate possessed bacteriostasis on Escherichia coli, Staphylococcus aureus and Salmonella. Animal
test results showed that the count of Salmonella in the feces of mice administered by gavage with the pepsin hydrolysate was
significantly reduced (P < 0.05) when compared with the positive group, whereas the count of Staphylococcus aureus in the
feces of mice orally administered with the ProteAX hydrolysate was significantly lower (P < 0.05) when compared with the
positive group.

Key words: enzymatic hydrolysate of sea buckthorn seed residue, bacteriostatic activity, in vivo and in vitro test