Abstract:

A novel method for the determination of gigantol in Dendrobii Caulis by dispersive liquid-phase microextraction
(DLPME) coupled with high performance liquid chromatography (HPLC) was developed. The optimal DLPME conditions
were found to be extraction by vortexing for 1 min using 120 μL of valeric acid as the extraction solvent and 80 μL of Triton
X-114 as the dispersant at pH 3, yielding an average 28.02-fold enrichment and an average recovery of 1.01 for gigantol.
The proposed method exhibited a limit of detection (LOD) of 2.625 μg/L, a limit of quantification (LOQ) of 8.74 μg/L and a
linear range of 0.10–30.00 mg/L. The method was successfully applied to analyze real samples, and the results showed that it
was a rapid and efficient method for the extraction of gigantol in Dendrobii Caulis.

Key words: DLLME, phenolic components, gigantol, Dendrobii Caulis