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Determination of Eight Organic Acids in Blueberries by HPLC

XU Yutao1, LI Keke1, WANG Hexin2, CHEN Lirong3, GONG Xiaojie1,*   

  1. 1. College of Medical, Dalian University, Dalian 116622, China; 2. Modernization of Agricultural Research Center, Dalian University,
    Dalian 116622, China; 3. College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, China
  • Online:2015-09-25 Published:2015-09-11
  • Contact: GONG Xiaojie

Abstract:

A high performance liquid chromatography (HPLC) method was established for the simultaneous determination of
8 organic acids in blueberry fruit extract. This method was then used to determine the contents of organic acids in blueberries
from 26 cultivars. Among the blueberry cultivars tested, the content of total organic acids (25.135 26 mg/g fresh fruit) was
highest in Sierra and lowest in Croaton (only 2.368 31 mg/g fresh fruit). This method is simple, reproducible, accurate,
reliable and useful for the analysis of organic acids in blueberries.

Key words: blueberry, organic acid, citric acid, high performance liquid chromatography (HPLC), quantification

CLC Number: