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Separation, Identification and Antioxidant Activity of Rare Flavonoid Sanggenon M in Black Mulberry Grown in Xinjiang

XIANG Wei1, YU Yan1, LIU Jing1, GU Shaowei1, XU Li1,*, ZUO Shaochun2, HUANG Xianzhi1, PU Bin2   

  1. 1. College of Biotechnology, Southwest University, Chongqing 400716, China;
    2. Hetian Sericultural Research Institute of Xinjiang Uygur Autonomous Region, Hetian 848000, China
  • Online:2015-11-15 Published:2015-12-03

Abstract:

A rare flavonoid compound was obtained from black mulberry branches through extraction with 70% cold
ethanol, fractionation by organic solvent extraction, column chromatography and thin-layer chromatography (TLC). The
flavonoid was identified as sanggenon M by spectroscopic and structural analysis. Antioxidant activity of sanggenon M was
evaluated in comparison with that of the synthetic antioxidant butylated hydroxytoluene (BHT). The IC50 of sanggenon M
for scavenging DPPH radical and hydroxyl radicals were 45.984 and 65.692 mg/L, respectively, compared to 64.189 and
231.556 mg/L for BHT. The total reducing power of sanggenon M was obviously higher than that of the control.

Key words: black mulberry branch, sanggenon M, separation and identification, flavonoid, antioxidant activity

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