Abstract:

Objective: To investigate the protective effect of purified polyphenols from Punica granatum L. leaves 2 (PPPL2)
on acute alcoholic hepatic injury in mice. Methods: Totally 60 mice were randomly divided into control group, model group,
low-, middle- and high-dose PPPL2 groups, and positive control group (bifendate, 150 mg/(kg·d)) of 10 mice each. The
mice from the control and model groups were administrated with deionized water (0.1 mL/(10 g·d)) daily by gavage for
30 consecutive days. The mice from three different dose groups were administrated with PPPL2 at 100, 200 and 400 mg/(kg·d)),
respectively. The mice from the positive control group were administrated with bifendate (150 mg/(kg·d)). The model of acute
alcoholic liver injury was established on the 31st day, and all mice were gavaged with 50% alcohol (12 mL/kg), except for the
control group. Biochemical indicators of ocular blood in mice were observed. Results: Compared with the model group, the levels
of glutamic-oxaloacetic transaminase (AST), alanine transaminase (ALT), and triglyceride (TG) were obviously decreased in the
treatment groups, and the mice from these treatment groups had reduced expansion of mitochondria and glycogen deposition. In
addition, the endoplasmic reticulum was not obviously outspread and lipid droplets were reduced in hepatic cells. Conclusion:
Polyphenols from pomegranate leaves have protective effect on alcoholic hepatic injury.

Key words: polyphenols from pomegranate leaves, acute alcoholic hepatic injury, biochemical indicators, ultrastructure