Abstract:

The extraction conditions of total flavonoids from Chrysanthemum coronarium L. leaves were optimized using
quadratic regression orthogonal rotary method. The flavonoids extracted were purified by preparative chromatography. Their
antioxidant activities were evaluated by four different methods. The optimal extraction conditions were determined as follows:
extraction temperature, 73 ℃; ethanol concentration, 68%; ratio of solvent to sample, 30:1; microwave power, 400 W; and
extraction time, 8 min. Under the optimal extraction conditions, the extraction rate of total flavonoids was 0.554%. The
flavonoids showed high total antioxidant activity, radical scavenging activity against hydroxyl and 1,1-diphenyl-2-picrylhydrazyl
radical (DPPH) free radicals, and inhibitory effect on lipid peroxidation of yolk lipoprotein.

Key words: flavonoids from Chrysanthemum coronarium L. leaves, quadratic regression orthogonal rotary method, preparative chromatography, antioxidant activity