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ZHANG Long, LIU Junrong*, TIAN Yuanyong, LIU Jinyang, WU Zhong, WANG Wei
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Abstract:
This study investigated quality parameters of soft body tissues of the clam Ruditapes philippinarum such as anatomic composition, proximate composition, and the distribution of nitrogenous compounds with emphasis on proteins. The results were indicated that 1) total edible portions accounted for about 20% of Ruditapes philippinarum. Proximate composition of the edible portions including six tissues, foot, adductor, mantle, siphon, gill and viscera, consisted of moisture (74.01%–80.07%), protein (29.19%–43.46%, dry basis), carbohydrate (12.46%–30.75%, dry basis), fat (1.61%–6.84%, dry basis) and ash (6.69%–11.10%, dry basis); 2) the nitrogenous components were distributed in non-protein nitrogen (14.7%– 26.42%), water-soluble protein (12.55%–19.17%), salt-soluble protein (34.83%–50.4%), and alkali-soluble protein (17.49%– 25.06%); and 3) sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that the protein patterns varied among the soft tissues and components, with the water-soluble proteins, salt-soluble proteins and alkalisoluble proteins distributed below 100 kD, near 200, 100, 45, 35 and 20 kD, and near 200, 100 and 45 kD, respectively, and the stroma proteins distributed in a wide range, mainly near 45 kD and at 200 kD.
Key words: Ruditapes philippinarum, soft tissues, non-protein nitrogen, protein, composition profiles
CLC Number:
TS254
ZHANG Long, LIU Junrong*, TIAN Yuanyong, LIU Jinyang, WU Zhong, WANG Wei. Composition and Distribution of Nitrogenous Compounds in Soft Body of Ruditapes philippinarum[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201524016.
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URL: https://www.spkx.net.cn/EN/10.7506/spkx1002-6630-201524016
https://www.spkx.net.cn/EN/Y2015/V36/I24/95