Abstract:

In this study, we aimed to predict the type of secondary metabolites of the endophyte Salicorn 3 isolated from
wild Salicornia bigelovii Torr. by using the type I polyketide synthase (PKS I) gene as a probe. As a result, two polyketide
compounds were isolated from the fermentation product of Salicorn 3. Based on the ITS1-5.8S-ITS4 rDNA sequence
analysis, the strain Salicorn 3 was identified as Penicillium cyclopium. The isolate was found to have the potential to produce
polyketide compounds by phylogenetic analysis and comparing the data with the reference. Two pure compounds were
obtained from the fermentation product of Salicorn 3 using open silica gel column chromatography and Sephadex LH-20,
and their chemical structures were elucidated as (1) ditryptophenaline and (2) cerebroside C by nuclear magnetic resonance
(NMR) and electrospray ionization mass spectrometry (ESI-MS).

Key words: Salicornia bigelovii Torr., endophytic fungi, type I PKS functional gene, polyketide compounds, nuclear magnetic resonance