Abstract:

Objective: To find out and expand the utilization potential of Phallus impudicus L. by analyzing the chemical
composition of petroleum ether and ethanol extracts from its pileus, stipe and volva. Methods: The volatile components of the
pileus, stipe and volva of Phallus impudicus L. were obtained by successive extraction with petroleum ether and ethanol under
room temperature (25–30 ℃). The chemical composition of each extract was identified by gas chromatography-mass spectrometry
(GC-MS) using a polar column HP-INNOWAX. Temperature programming was as follows: initial temperature was set to 60 ℃
hold for 2 min, followed by an increase of 10 ℃/min to 160 ℃ held for 6 min, and another increase of 3 ℃/min to 220 ℃ held
for 17 min. The spectra were compared with those published in the mass spectral library NIST 08. Results: A total of 67 volatile
components were identified from the two different solvent extracts of Phallus impudicus L., 66 compounds of which were first
reported in this study. A total of 35 compounds were extracted with petroleum ether from Phallus impudicus L., 24, 18 and 18
of which were identified from the extracts of pileus, stipe and volva, respectively. A total of 38 components were extracted from
Phallus impudicus L. including 26, 28 and 12 compounds identified from the extracts of pileus, stipe and volva, respectively.
Conclusions: The presence of 9,12-octadecadienoic acid (Z,Z)-, butylated hydroxytoluene, 1,2-benzenedicarboxylic acid, bis
(2-methylpropyl) ester, isosorbide, 1,2-cyclopentanedione, 2-pyrrolidinone, niacinamide, 9,12-octadecadienoic acid, and ethyl ester
can provide the scientific basis for reasonable utilization of Phallus impudicus L..

Key words: Phallus impudicus L., petroleum ether extract, ethanol extract, gas chromatography-mass spectrometry (GC-MS)