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Separation and Identification of Chemical Constituents from Garcina oblongifolia Fruits

LI Ping1, GUO Zhiyong1, GU Ronghui1, ZHANG Beixi1, WU Xiaohuan1, LI Yuting1, LONG Chunlin1,2,*   

  1. 1. College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China;
    2. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Online:2016-03-25 Published:2016-03-18

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Abstract:

High performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-TOF-MS) was
applied to analyze the chemical composition of Garcina oblongifolia fruits. The crude extract of Garcina oblongifolia fruits
was separated and purified by sequential chromatographies using silica gel column, Sephadex LH-20 column, high speed
counter current chromatograhy (HSCCC). The structures of the purified compounds were confirmed by nuclear magnetic
resonance (NMR). As a result, 11 compounds, which were mainly categorized to benzophenones and biflavonoids, were
tentatively identified from the fruit extract. Further isolation yielded 5 compounds, which were identified as oblongifolin C (1),
oblongifolin A (2), volkensiflavone (3), GB-1a (4) and β-sitosterol (5), respectively, of which compounds 3 and 4 were isolated
from this plant for the first time. This study provides important evidence supporting the exploitation of this tropical fruit.

Key words: Garcina oblongifolia fruits, benzophenones, biflavonoids, high performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-TOF-MS)

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