Abstract:

An improved method for the determination of phospholipids in caviar by solution 31P-nuclear magnetic
resonance (31P-NMR) was studied. The lipids in the sample were extracted with chloroform and methanol, dissolved in
deuterated reagent and then analyzed by 31P-NMR without separation. The average recoveries of the established method
were 96%–102%, with relative standard deviation (RSD) not exceeding 5%. Phospholipids of 4 commercial brands of
caviar were analyzed and the relative distribution of phospholipid components was measured in mole percentage. It was
found that phosphatidylcholine and lysophosphatidylcholine accounted for more than 80% of the total phospholipids in
lumpfish roe, and phosphatidylinositol content in sturgeon roe was much higher than that of lumpfish roe. The contents of
phosphatidylethanolamine, sphingomyelin and phosphatidic acid were similar in both fish roes.

Key words: 31P-nuclear magnetic resonance (31P-NMR), phospholipids, caviar, determination