Abstract:

Objective: To establish a high performance liquid chromatography (HPLC) method for the simultaneous
determination of syringic acid, ferulic acid, rutin, daidzein, quercetin, quercetin, quercetin and quercetin in Herba Leonuri.
Methods: The analytes were separated on an InertSustain C18 (150 mm × 4.6 mm, 5 μm). The mobile phase consisted of
methanol and 0.1% acetic acid (pH 3.0) with gradient elution at a flow rate of 1.0 mL/min. The UV detection wavelength
was set at 285 nm. The column temperature was maintained at 35 ℃. The injection volume was 20 μL. Results: The eight
compounds were well separated, showing good linear correlations with correlation coefficient (r) ≥ 0.999 1 (n = 7). The
average recoveries were 89.93%–102.01%, with relative standard deviations (RSDs) between 1.17% and 3.11%. Conclusion:
The method is simple, rapid and accurate, which can provide an experimental basis for quality control of Herba Leonuri.

Key words: high performance liquid chromatography (HPLC), Herba Leonuri, flavonoids, organic acid, quantification