Antioxidant Activity and Preliminary Structure Analysis of Polysaccharides from Cordyceps cicadas

doi:10.7506/spkx1002-6630-201613004

Abstract

Abstract:

In this study, the antioxidant activities and structural characteristics of polysaccharides from Cordyceps cicadae
were determined. Two crude polysaccharide fractions (CP50 and CP80) were extracted from Cordyceps cicadae with hot
water and isolated by precipitation with different concentrations of ethanol. Their antioxidant activities were also analyzed.
The results indicated that both CP50 and CP80 were effective in antioxidant activity. The radical scavenging activity of
CP50 was higher than that of CP80. CP50 also showed higher reducing capacity and total antioxidant activity. CP50 was then
further purified by gel filtration on diethylaminoethyl (DEAE)-cellulose-52 and Sephadex G-100 columns, and two purified
fractions were obtained, namely CPA-1 and CPB-1. Ultraviolet spectroscopy and high-performance liquid chromatography
(HPLC) analysis revealed that CPA-1 and CPB-1 were homogeneous. Monosaccharide analysis revealed that both CPA-1
and CPB-1 were composed of Glc, Gal and Man with a molar ratio of 1:0.48:0.52 and 1:0.14:0.114, respectively. Moreover,
both polysaccharide fractions had three helix structures.

Key words: Cordyceps cicadaes polysaccharides, antioxidant activity, separation and purification, structural analysis

CLC Number:

R284.1

FENG Yan, GONG Xiaohui, WEI Dequn, LIU Yingkun, ZHAO Ming, YU Xiaofeng, OPEYEMI Joshua Olatunji,JIAO Xinyi, OUYANG Zhen. Antioxidant Activity and Preliminary Structure Analysis of Polysaccharides from Cordyceps cicadas[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201613004.

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