Optimization of Disruption of Ganoderma lucidum Spores and Antitumor Effect of the Broken Spores

doi:10.7506/spkx1002-6630-201614009

Abstract

Abstract:

The purpose of this study was to investigate the optimum conditions for breaking Ganoderma lucidum spores
by high-pressure homogenization using water as the solvent and to evaluate the antitumor effect of the broken spores.
The optimization of three process variables including solid-to-liquid ratio, homogenization pressure, and the number
of homogenization cycles for improved percentage of broken spores was conducted through one-factor-at-a-time and
orthogonal array experiments. S180 tumor-bearing mice models were established for evaluation of the antitumor effect of
the broken G. lucidum spores. The results showed that the optimal conditions for breaking G. lucidum spores were obtained
as follows: homogenization pressure, 150 MPa; homogenization cycles, 3 times; and solid-to-liquid ratio, 1:100 (g/mL).
Under these conditions, the percentage of broken spores could be up to 94.35%. Antitumor tests showed that the percentage
inhibition of tumor growth in S180 tumor-bearing mice by the broken spores was 43.37%–57.59%, and the liver index and
lung index were little affected while the thymus index and spleen index were significantly enhanced. Therefore, broken G.
lucidum spores possessed good antitumor effect.

Key words: Ganoderma lucidum spores, spore disruption, high pressure homogenization method, antitumor effect

CLC Number:

R285.5

LIU Chunyan, ZHANG Guocai, CHENG Fangzhi, ZHANG Guozhen, ZHAO Bo, LIN Liannan. Optimization of Disruption of Ganoderma lucidum Spores and Antitumor Effect of the Broken Spores[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201614009.

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