Development of EGCG Reference Material

doi:10.7506/spkx1002-6630-201614019

Abstract

Abstract:

Objective: This study aimed to prepare epigallocatechin gallate (EGCG) reference material according to the
Directives for the Work of Reference Materials (3): Reference Materials—General and Statistical Principles for Certification
(GB/T 15000.3—2008). Methods: EGCG from green tea was separated by extraction and high speed counter current
chromatography (HSCCC). Its chemical structure was identified by ultraviolet-visible (UV) spectroscopy, infrared (IR)
spectroscopy, mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. The homogeneity and stability
were systematically checked. A cooperative validation was conducted in 8 laboratories and the results of certification for
EGCG were analyzed. Results: The homogeneity and stability of the reference material of EGCG were excellent, and the
certified value was 99.29% with an expanded uncertainty of 0.16% at confidence coefficient of 95%. Conclusions: The
reference material of EGCG conforms to the requirement of the Directives for the Work of Reference Materials (3) and can
be used for validation of analytical methods for quality control of green tea products.

Key words: epigallocatechin gallate (EGCG), green tea, certified reference material, homogeneity, stability, certified value

CLC Number:

TS272.7

WANG Wei, LIN Nan, ZHOU Xiaojing, LE Shengfeng, DU Ning, ZHANG Jinghua. Development of EGCG Reference Material[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201614019.

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