Simultaneous Determination of Cholesterol and Phytosterol in Foods by Gas Chromatography

doi:10.7506/spkx1002-6630-201614032

Abstract

Abstract:

A method for the simultaneous determination of phytosterol and cholesterol in foods was established by gas
chromatography (GC) in this research. Cholesterol, brassicasterol, campesterol, stigmasterol, β-sitosterol, and stigmasterol
were quantified using 5α-cholestane as the internal reference standard. Samples were saponified before extraction and the
extract was concentrated and diluted again with n-heptane before being injected into the chromatographic system. The results
showed that cholesterol and phytosterols were separated well and displayed linear relationship over the concentration range
of 0.001–2.5 mg/mL. The limit of detection (LOD) and limit of quantitation (LOQ) of this method were 0.3 and 1.0 mg/100 g,
respectively. Compared with high performance liquid chromatography (HPLC), the GC method could effectively separate
cholesterol and phytosterols, with simpler operation, better reproducibility, and higher accuracy and sensitivity.

Key words: gas chromatography, cholesterol, phytosterol

CLC Number:

TS207.3

CHEN Yuexiao, HE Tao, TANG Lingxuan, XU Jiajia, BAI Shasha, CUI Yajuan, HE Mei, LI Dong, HUANG Hua, LU Yong. Simultaneous Determination of Cholesterol and Phytosterol in Foods by Gas Chromatography[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201614032.

References

[1] 韩军花, 杨月欣. 植物甾醇的性质、功能及应用[J]. 国外医学(卫生

学分册), 2001, 28(5): 285-291.

[2] 周晓星, 苏宜香. 植物甾醇降胆固醇作用的研究现状[J]. 国外医学

(卫生学分册), 2007, 34(2): 101-105.

[3] OSTLUND R E J. Phytosterols in human nutrition[J]. Annual Review

of Nutrition, 2002, 22: 533-549. DOI:10.1002/jssc.200900180.

[4] MIETTINEN T A, PUSKA P, GYLLING H, et al. Reduction

of serum cholesterol with sitostanol-estermargarine in a mildly

hypercholesterolemic population[J]. New England Journal of Medicine,

1995, 333: 1308-1312. DOI:10.1056/NEJM199511163332002.

[5] NIGON F, SERFATY-LACROSNIERE C, BEUCLER I, et al. Plant

sterol-enriched margarine lowers plasma LDL inhyperlipidemic

subjects with low cholesterol intake: effectof fibrate treatment[J].

Clinical Chemistry and Laboratory Medicine, 2001, 39(7): 634-640.

[6] 左玉. 植物甾醇研究与应用[J]. 粮食与油脂, 2012, 25(7): 1-4.

DOI:10.3969/j.issn.1008-9578.2012.07.001.

[7] 朱俊东, 糜漫天. 植物甾醇降低血胆固醇作用的研究进

展[J]. 重庆医学, 2006, 35(19): 1798-1800. DOI:10.3969/

j.issn.1671-8348.2006.19.039.

[8] 张艳, 张丽, 王俊芳. 植物甾醇对预防血管动脉硬化的作用[J]. 西北

国防医学杂志, 2012, 33(4): 466-468.

[9] 张蕾, 陈庆森, 闫亚丽, 等. 植物甾醇对心血管疾病作用的研究现状[J].

食品科学, 2013, 34(23): 344-350. DOI:10.7506/spkx1002-6630-

201323069.

[10] BERND G, GUNTHER S, de GUY B, et al. Plant sterols and

cardiovascular disease: a systematicreview and meta-analysis[J].

European Heart Journal, 2012, 33(4): 444-451.

[11] AGOT L, AMUNDSEN P, LEIV O, et al. Plant sterol ester-enriched

spread lowers plasma total and LDL cholesterol in children with

familial hypercholesterlemia[J]. American Journal of Clinical Nurition,

2002, 76(2): 338-344.

[12] 谢心美, 郝海鑫, 何剑斌. 植物甾醇的生理功能及其应用[J]. 草业科

学, 2013, 30(12): 2105-2109.

[13] 韩军花, 杨月欣, 冯妹元, 等. 中国常见植物食物中植物甾醇

的含量和居民摄入量初估[J]. 卫生研究, 2007, 36(3): 301-305.

DOI:10.3969/j.issn.1000-8020.2007.03.012.

[14] 冯妹元, 韩军花, 刘成梅, 等. 常见精练油中植物甾醇测定方法

的建立及含量分析[J]. 中国食品卫生杂志, 2006, 18(3): 197-201.

DOI:10.3969/j.issn.1004-8456.2006.03.002.

[15] 彭浩, 陈文强, 邓百万. 气相色谱法测定食用菜籽油中植物甾

醇的组成及含量[J]. 安徽农业科学, 2006, 34(19): 4830-4831.

DOI:10.3969/j.issn.0517-6611.2006.19.005.

[16] 王三永, 李晓光, 李春荣, 等. 含脂肪食品中植物甾醇含量的气相

色谱法测定[J]. 分析测试学报, 2001, 20(4): 43-44. DOI:10.3969/

j.issn.1004-4957.2001.04.013.

[17] 高瑜莹, 裘爱泳, 潘秋琴, 等. 植物甾醇的分析方法[J]. 中国油脂,

2001, 26(1): 25-28. DOI:10.3321/j.issn:1003-7969.2001.01.011.

[18] 杨虹, 姜元荣, 魏婷婷, 等. 食用油中植物甾醇测定方法的优化及含

量分析[J]. 中国粮油学报, 2011, 26(2): 120-123; 128.

[19] 李和, 李玉帼. 植物油中常见的几种植物甾醇的薄层色谱分离与鉴

别[J]. 中国油脂, 1997, 22(2): 20-22.

[20] 陈茂彬, 吴谋成. 植物甾醇的毛细管气相色谱分析方法

研究[J]. 中国粮油学报, 2004, 19(6): 88-90. DOI:10.3321/

j.issn:1003-0174.2004.06.024.

[21] Association of Official Analytical Chemists. AOAC official method

2007.03 campesterol, stigmasterol, and beta-sitosterol in saw palmetto

raw materials and dietary supplements gas chromatography first action

2007[S].

[22] Association of Official Analytical Chemists. AOAC official method

994.10 cholesterol in foods direct saponification-gas chromatographic

method first action 1994[S].

[23] 薛芳, 戴亚, 李东亮, 等. 烟草中植物甾醇的GC-MS分析[J]. 化学研

究与应用, 2012, 24(4): 617-620.

[24] 杨春英, 刘学铭, 陈智毅, 等. 气相色谱-质谱联用法测定14 种食

用植物油中的植物甾醇[J]. 2013, 28(2): 123-128. DOI:10.3969/

j.issn.1003-0174.2013.02.026.

[25] 徐响, 孙丽萍, 董捷, 等. 气相色谱-质谱法测定蜂王浆中的植物甾醇

组成[J]. 食品科学, 2010, 31(18): 317-320.

[26] 张红城, 董捷, 任向楠, 等. 四种蜂花粉中植物甾醇的GC-MS分析[J].

2008, 29(12): 522-524. DOI:10.3321/j.issn:1002-6630.2008.12.118.

[27] TOIVO J, PHILLIPS K, LAMPI A M, et al. Determination of sterols in

foods: recovery of free, esterified, and glycosidic sterols[J]. Journal of

Food Composition and Analysis, 2001, 14(6): 631-643. DOI:10.1006/

jfca.2001.1019.