FOOD SCIENCE

• Bioengineering • Previous Articles     Next Articles

Correlation between 1-Deoxynojirimycin Content and Glucosidase Activities in Silkworm (Bombyx mori L.)

CHEN Hongxia1,2, LIU Yueqin2, PAN Gang3, ZHAO Ming2, OUYANG Zhen1,2,*   

  1. 1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; 2. School of Pharmacy, Jiangsu University, Zhenjiang 212013, China; 3. The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China
  • Online:2016-08-15 Published:2016-08-30

RichHTML

2

PDF (PC)

251

Abstract:

In order to reveal the mechanism of 1-deoxynojirimycin (DNJ) enrichment in silkworm (Bombyx mori L.), we
studied and analyzed the correlation between DNJ content and glucosidase activities in silkworm. Towards this goal, 25
strains of silkworms were selected and raised with the same mulberry leaves. Pre-column derivatization high performance
liquid chromatography (HPLC) was applied to determine the DNJ content of different strains of silkworms, and the activities
of 3 major glucosidases (sucrase, lactase and amylase) were measured, respectively, for the analysis of correlation between
DNJ content and glucosidase activities in silkworms. Our results indicated that different strains of silkworms had significant
differences in DNJ content, and the highest DNJ content (which was found in the strain 2042, 0.56%) was 7 times higher
than the lowest one (in ‘DongTing × BiBo’, 0.08%). DNJ content had significantly negative correlations with the activity of
either of two disaccharidases, sucrase and lactase, and had a relatively weaker correlation with amylase activity.

Key words: Bombyx mori L., 1-deoxynojirimycin, glucosidase, correlation

CLC Number: