Establishment of a High-Throughput Fluorescence Multiplex PCR Method for Detection of Genetically Modified Rice Events

doi:10.7506/spkx1002-6630-201620032

FOOD SCIENCE ›› 2016, Vol. 37 ›› Issue (20): 187-190.doi: 10.7506/spkx1002-6630-201620032

• Safety Detection • Previous Articles Next Articles

Establishment of a High-Throughput Fluorescence Multiplex PCR Method for Detection of Genetically Modified Rice Events

ZHANG Mingzhe, ZHANG Xiaofeng, XU Lili, CHEN Xiaomei, ZHOU Yuan, YIN Wenxiu, CHEN Wujian, LI Xiaojun

1. Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou 310016, China;
2. Zhoushan Entry-Exit Inspection and Quarantine Bureau, Zhoushan 316000, China

Received:2016-10-27 Revised:2016-10-27 Online:2016-10-25 Published:2016-12-01

Abstract

Abstract:

In this research, a high-throughput and specific method for the detection of genetically modified (GM) rice events
was developed using fluorescence multiplex PCR and capillary electrophoresis based on fluorescence-labeled primers for
endogenous, exogenous and event-specific genes. The fluorescence multiplex PCR method could specifically detect four
GM rice events with a sensitivity of 0.1%. This method has the potential to be applied in the inspection and quarantine of
import and export genetically modified commodities and in food safety detection.

Key words: multiplex PCR, capillary electrophoresis, genetic modified rice, identification of genetically modified events

CLC Number:

Q789h-

ZHANG Mingzhe, ZHANG Xiaofeng, XU Lili, CHEN Xiaomei, ZHOU Yuan, YIN Wenxiu, CHEN Wujian, LI Xiaojun. Establishment of a High-Throughput Fluorescence Multiplex PCR Method for Detection of Genetically Modified Rice Events[J]. FOOD SCIENCE, 2016, 37(20): 187-190.

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Establishment of a High-Throughput Fluorescence Multiplex PCR Method for Detection of Genetically Modified Rice Events

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