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Separation of Major Royal Jelly Protein 1 Using Different Hydrophobic Chromatographic Columns

WANG Xueyu1, QIAO Jiangtao1, LIU Yinkang1,2, ZHANG Hongcheng1,3, DONG Jie1,3,*   

  1. 1. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China;
    2. College of Food Science, Harbin University of Commerce, Harbin 150076, China;
    3. National Research Center of Bee Product Processing, Ministry of Agriculture, Beijing 100093, China
  • Online:2017-01-15 Published:2017-01-16

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Abstract: To make a comparison of different hydrophobic chromatography columns for separating major royal
jelly protein 1 (MRJP 1), and choose a suitable one to separate MRJP 1 oligomer and MRJP 1 monomer. Methods: Totally 12
kinds of hydrophobic chromatography columns were applied to separate crude royal jelly protein, then the MRJP 1 oligomer
and MRJP 1 monomer fractions were verified by native gel electrophoresis. Results: MRJP 1 was completely isolated by
hydrophobic interaction chromatography on Hitrap Butyl HP, and an MRJP 1 oligomer fraction and an MRJP 1 monomer
fraction were obtained. Conclusion: Hydrophobic chromatographic column provides an effective approach to separate
MRJP 1 from royal jelly.

Key words: major royal jelly protein 1, hydrophobic chromatography column, native gel electrophoresis, protein separation

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