Purification and Identification of Antibacterial Peptides from Cottonseed Protein Isolate Hydrolysates

doi:10.7506/spkx1002-6630-201711020

Abstract

Abstract: Cottonseed protein isolate (CPI) was digested in vitro to prepare hydrolysates with antibacterial activity. Ultrafiltration (UF), anion exchange chromatography (AEC), and semi preparative high performance liquid chromatography (semi-P-HPLC) were used to isolate and purify antibacterial peptides from CPI hydrolysates, and the purified peptides were sequenced by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). During the purification process, CPI hydrolysates were separated into three fractions: U-Ⅰ, U-Ⅲ and U-III by UF and the obtained U-Ⅲ, with higher antibacterial ability, was further separated into QF-Ⅰ, QF-Ⅱ and QF-Ⅲ by AEC. After filtration, QF-Ⅱ, which showed higher antibacterial ability, was further fractionated using semi-P-HPLC into four subfractions: PF-Ⅰ, PF-Ⅱ, PF-Ⅲ and PF-Ⅳ, among which, PF-Ⅲ was determined to have the highest antibacterial activity. The purity of PF-Ⅲ showed a single peak in HPLC. Finally, by ESI-MS/MS, and the amino acid sequence of the peptide was identified as ISGLIYEETR (Ile-Ser-Gly-Leu-Ile-Tyr-Glu-Glu-Thr-Arg).

Key words: cottonseed protein isolate hydrolysates, peptide with antibacterial activity, isolation and purification, amino acid sequence

CLC Number:

TS201.3

HAN Xiaoyan, BAO Yuming, XIN Fengjiao, HUANG Ying, Christophe BLECKER, DAI Xiaofeng. Purification and Identification of Antibacterial Peptides from Cottonseed Protein Isolate Hydrolysates[J]. FOOD SCIENCE, 2017, 38(11): 122-127.

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Purification and Identification of Antibacterial Peptides from Cottonseed Protein Isolate Hydrolysates

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