A Label-Free Immunoassay with a Surface Plasmon Resonance Biosensor for the Determination of Estradiol in Milk

doi:10.7506/spkx1002-6630-201712034

Abstract

Abstract: A surface plasmon resonance (SPR) biosensor system was developed for the determination of estradiol (E2) using an indirect competition method without labeling in real time. In the indirect competition method, the antigen (E2-BSA) was immobilized on the sensor chip surface and competed with E2 in samples for binding with the antibodies. Then the SPR response decreased in the presence of E2, which prevented the coupling of antibodies and E2-BSA, and so the SPR response was inversely proportional to E2 concentration. Assay parameters, including antibody concentration and number of regenerations, were optimized and E2-spiked milk samples were detected by this method. A linear relationship was obtained between inhibition percentage and common logarithmic value of E2 concentration over the range of 15.63 to 500 ng/mL and the limit of detection (LOD) was 11.13 ng/mL. The present study demonstrated a simple and general strategy for label-free detection of various small molecules.

Key words: surface plasmon resonance (SPR), label-free, estradiol, real-time, indirect competition

CLC Number:

X836

JIA Yingtong, PENG Yuan, BAI Jialei, ZHANG Xihao, NING Bao’an, GAO Zhixian, CUI Jiansheng. A Label-Free Immunoassay with a Surface Plasmon Resonance Biosensor for the Determination of Estradiol in Milk[J]. FOOD SCIENCE, 2017, 38(12): 223-228.

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A Label-Free Immunoassay with a Surface Plasmon Resonance Biosensor for the Determination of Estradiol in Milk

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