FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (22): 317-321.doi: 10.7506/spkx1002-6630-201722047

• Safety Detection • Previous Articles     Next Articles

Simultaneous Determination of Cefotaxime and Its Main Metabolite in Chicken Plasma by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

YANG Xiaoti, TANG Xiaoyan,, ZHANG Xiaoqing, SHEN Xixi   

  1. (1. Key Laboratory of Meat Processing and Quality Control, Ministry of Education, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. Key Laboratory of Agri-Food Safety and Quality, Ministry of Agriculture,Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
  • Online:2017-11-25 Published:2017-11-03

Abstract: In order to study the pattern of cefotaxime metabolism in chicken plasma, an ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was successfully developed for the simultaneous determination of cefotaxime and its metabolite desacetylcefotaxime. Cefotaxime at a dose of 10 mg/kg was administered to laying hen, and chicken plasma was collected at different time points to quantitate cefotaxime and desacetylcefotaxime by UPLC-MS/MS. The results showed that the limits of detection (LOD) for cefotaxime and desacetylcefotaxime were 0.07 and 0.14 μg/L, respectively; the limits of quantification (LOQ) were 0.23 and 0.99 μg/L, respectively. The recoveries of cefotaxime and desacetylcefotaxime ranged from 86.7%–95.2% and 89.5%–112%, respectively, with relative standard deviation (RSD) less than 7.7%, which met the requirements for quantitative analysis. Cefotaxime was rapidly distributed in laying hens and reached the peak (56.34 mg/L) immediately after administration, and the rate of cefotaxime metabolism was fast, which was detected at a level lower than 3.0 μg/L after 24 hours. The main metabolite deacetylcefetaxime reached its peak (10.22 mg/L) after 45 min, and it was eliminated at a relatively slow rate was and was still as high as 54.0 μg/L at 24 h after administration.

Key words: cefotaxime (CTX), desacetylcefotaxime (DCTX), ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), plasma

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