Abstract: Objective: To establish a method for the determination of β-phenylethanol in rice wine by solid phase extraction (SPE)-gas chromatography (GC). Methods: β-Phenylethanol was enriched from 3.0 mL of rice wine with an activated and balanced HLB solid phase extraction column, which was washed with 3.0 mL of water and 3.0 mL of methanol-water (4:6, V/V), and eluted with 3.0 mL of methanol. A gas chromatograph equipped with flame ionization detector (GC-FID) was used to analyze the target compounds. Results: In the concentration range of 10.1–202.0 mg/L, good linearity was achieved with a correlation coefficient (r) of 0.999 9. The limit of detection (LOD, RSN = 3) and the limit of quantification (LOQ, RSN = 10) were 0.9 and 3.0 mg/L, respectively. The recovery of β-phenylethanol was in the range of 95.5%?98.5%. The precision values expressed as relative standard deviations (RSDs) were 1.1%?1.4%. Conclusion: The method is simple, convenient, accurate and applicable to the determination of β-phenylethanol in rice wine and can effectively reduce the pollution of the gas chromatography system by rice wine matrix and prevent water in rice wine samples from bringing harm to the capillary column.

Key words: solid phase extraction (SPE), gas chromatography (GC), rice wine, β-phenylethanol