Development and Application of Pork Traceability System Based on SNP Markers

doi:10.7506/spkx1002-6630-201724045

Abstract

Abstract: DNA traceability technology is based on DNA sequence differences between individuals for individual identification and the tracing of meat back to an individual animal. Establishing a DNA-based traceability system is important for the safety and quality of meat products. The genetic diversity of 33 new single nucleotide polymorphism (SNP) markers was detected in a test group (233 individuals, 10 varieties) and 6 SNP markers, which are useful for pork product traceability, were obtained by heterozygosity calculation. A total of 18 SNP markers (6 new SNP markers in combination with 12 SNP markers) could effectively distinguish between 100 individuals collected from a local slaughter house in traceability simulation experiments. The corresponding individuals for tissue samples from 10 random individuals were found by genotype. This study has shown that SNP markers could be used for individual identification and pork traceability.

Key words: pork traceability, DNA traceability technology, SNP markers, individual identification

CLC Number:

Q819

WU Xiao, Lü Beibei, WANG Jinbin, JIANG Wei, LI Peng, WU Guogan, TANG Xueming. Development and Application of Pork Traceability System Based on SNP Markers[J]. FOOD SCIENCE, 2017, 38(24): 278-282.

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Development and Application of Pork Traceability System Based on SNP Markers

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