Abstract: In order to investigate the carbon source utilization profile of Shewanella putrefaciens at different temperatures, a kinetic model describing the growth of S. putrefaciens in multi-well plates at 5, 15, 25 and 33 ℃ was developed using modified Gompertz equation and the kinetic parameters maximum specific growth rate (μmax) and lag phase duration (λ) were obtained. Moreover, based on average well color development (AWCD) and utilization area, the carbon utilization rate of S. putrefaciens was analyzed. The results showed that S. putrefaciens presented the highest μmax and lowest λ at 25 ℃. Moreover, at this temperature, the highest carbon utilization and cell viability were observed followed by growth at 15 ℃; the cell viability at 5 ℃ was one-sixth of that at 25 ℃, and the lowest cell viability was found at 33 ℃. Temperature in the range of 5?25 ℃ exhibited a good linear relationship with and . The utilization rates of of carbohydrates and carboxylic acids were 30% and 29% at 5?33 ℃, respectively. Carbohydrates (dextrin, D-maltose, α-D-glucose, D-arabinol and stachyose), carboxylic acids (L-malic acid, L-lactic acid and acetoacetic acid), amino acids (L-serine, amino-acetyl-L-proline, L-aspartic acid and L-glutamic acid), fatty acids/lipids, amines/amides and other substances (gelatin, methyl pyruvate, Tween 40 and L-histamine) could be better utilized by the strain.

Key words: Shewanella putrefaciens, carbon, growth kinetics, multi-well plate