Establishment of Activity Assays for α-Glucosidase and Dipeptidyl Peptidase IV Using Porcine Small Intestine Mucosal Extracts

doi:10.7506/spkx1002-6630-20180615-294

FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (23): 44-51.doi: 10.7506/spkx1002-6630-20180615-294

• Basic Research • Previous Articles Next Articles

Establishment of Activity Assays for α-Glucosidase and Dipeptidyl Peptidase IV Using Porcine Small Intestine Mucosal Extracts

JIA Chengli, Naveed HUSSAIN, DONG Jie, WANG Fen, LI Hantong, ZHANG Shuwen, LU Jing, PANG Xiaoyang, LIU Lu, Lü Jiaping

(1. Key Laboratory of Agro-products Processing, Ministry of Agricultural and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. College of Food Science and Engineering, Shanxi Agricultural University, Jinzhong 030800, China; 3. College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; 4. Engineering Research Centre of System-Nutrition, Beijing Institute of Nutritional Resources, Beijing 100069, China)

Online:2019-12-15 Published:2019-12-24

Abstract

Abstract: Alpha-glucosidase and dipeptidyl peptidase IV (DPP-4) are two important target enzymes useful in the treatment of type II diabetes. Currently, commercially available enzymes are mainly obtained from microorganisms, making it difficult to screen for inhibitors of the enzymes. Both enzymes are widespread in mammalian small intestines. In this study, fresh porcine small intestinal mucosal secretions and epithelial cells were collected for comparative measurement of α-glucosidase and DPP-4 activities in mucosal extracts from three different intestinal segments. The extracts were used as reaction system of α-glucosidase and DPP-4 and their percentage inhibition by acarbose and diprotin A (IPI) respectively was evaluated. The median inhibition concentration (IC50) against commercial and porcine ileum-derived α-glucosidase was 1.102 4 and 0.244 7 mg/mL for acarbose, and 19.119 7 and 41.268 4 μg/mL for IPI, respectively. The specific activities of α-glucosidase and DPP-4 in porcine ileum mucosal extract were 0.084 1 and 0.053 4 U/mg, which were higher than those in the two other mucosal extracts. Therefore, porcine ileum mucosal extract can be used as an alternative to commercial α-glucosidase and DPP-4 for screening for enzyme inhibitors.

Key words: α-glucosidase, dipeptidyl peptidase IV (DPP-4), porcine intestine, specific enzyme activity

CLC Number:

TS201.2

JIA Chengli, Naveed HUSSAIN, DONG Jie, WANG Fen, LI Hantong, ZHANG Shuwen, LU Jing, PANG Xiaoyang, LIU Lu, Lü Jiaping. Establishment of Activity Assays for α-Glucosidase and Dipeptidyl Peptidase IV Using Porcine Small Intestine Mucosal Extracts[J]. FOOD SCIENCE, 2019, 40(23): 44-51.

[1]	XU Xiaomei, WEN Jiaying, WANG Qinghua. Molecular Docking of Small-Molecule Monosaccharides and Their Analogues with α-Glucosidase [J]. FOOD SCIENCE, 2020, 41(8): 139-143.
[2]	HUANG Qinqin, TIAN Yaping. α-Glucosidase and Dipeptidyl Peptidase IV Inhibitory Activity and Peptide Composition of Porphyra yezoensis Protein Hydrolysate [J]. FOOD SCIENCE, 2020, 41(24): 110-116.
[3]	HOU Can, DU Yuguang, WANG Xi，XIAO Jie, FAN Yihang, DONG Zhizhong, CHANG Guosheng, WANG Wei, LI Song, YING Jian. Fermented Dark Tea with Pericarpium Citri Reticulatae: Comparison of Chemical Composition with Raw Dark Tea and Bioactivities in Vitro [J]. FOOD SCIENCE, 2020, 41(18): 226-232.
[4]	GUO Shiyin, LI Lin, ZHOU Hong, TANG Zhonghai, SU Xiaojun, LI Qingming. Mechanism for the Inhibitory Effect of 1-Deoxynojirimycin on α-Glucosidase [J]. FOOD SCIENCE, 2019, 40(5): 45-50.
[5]	YAN Fenfen, SHI Jialu, LI Na, YUE Yingxue, LI Huizhen, SONG Yue, HUO Guicheng. YAN Fenfen, SHI Jialu, LI Na, YUE Yingxue, LI Huizhen, SONG Yue, HUO Guicheng [J]. FOOD SCIENCE, 2019, 40(20): 152-158.
[6]	HAN Fenxia, FAN Xinjing, GENG Sheng, LOU Wenjuan, LIANG Guizhao, LIU Benguo. Inhibitory Mechanism of Isoliquiritigenin against α-Glucosidase [J]. FOOD SCIENCE, 2019, 40(15): 37-42.
[7]	JIANG Lili, ZHANG Zhongmin, CHEN Daoyu, WANG Zhen, XUE Hongyu, LIU Yong. Inhibition Kinetics and Mechanisms of Resveratrol on α-Glucosidase [J]. FOOD SCIENCE, 2019, 40(11): 70-74.
[8]	ZHANG Lu, HUANG Xiangxia, TU Zongcai, ZHAO Yi, WANG Hui, WANG Hao, SHA Xiaomei, ZHANG Shuya. Comparison of Bioactive Compounds, Antioxidant Activity and α-Glucosidase Inhibition Activity of Five By-Products from Lotus Seed Production [J]. FOOD SCIENCE, 2018, 39(9): 33-38.
[9]	JING Yongshuai, ZHANG Ruijuan, WU Lanfang, SU Lei, LI Chao, ZHANG Danshen. Preparation, Structural Characteristics and Bioactivity of Polysaccharide-Iron Complex from Radix Glehniae [J]. FOOD SCIENCE, 2018, 39(24): 65-70.
[10]	YAN Hui, ZHANG Qi, JIANG Mingzhu, ZHU Shenghu, NIE Xudong, YU Yongjian, ZHANG Jiaxin, JIA Junqiang, XIONG Meng. Isolation and Structural Identification of Hypoglycemic Peptides from Wheat Germ Protein [J]. FOOD SCIENCE, 2018, 39(20): 92-98.
[11]	HAO Mengmeng, CUI Hanzhao, HAN Aizhi, YANG Ling. Extraction of Active Substances from Leaves of Morus nigra L. and Evaluation of Their Inhibitory Effect on α-Glycosidase [J]. FOOD SCIENCE, 2018, 39(19): 19-23.
[12]	WANG Fen, LIU Lu, LI Hantong, ZHANG Shuwen, LU Jing, PANG Xiaoyang, WANG Jianming, Lü Jiaping. Screening for Potential Probiotics Based on High α-Glucosidase Inhibitory Activity [J]. FOOD SCIENCE, 2018, 39(16): 192-200.
[13]	YU Ying, FAN Jinling, CHENG Yuanbin, ZHU Wenxue. Screening and Identification of α-Glucosidase Inhibitor in Waste Liquid from Glycyrrhizic Acid Extraction [J]. FOOD SCIENCE, 2018, 39(13): 22-28.
[14]	CAO Longkui, KANG Lijun, KOU Fang, SHEN Meng, GE Yunfei, WANG Weihao. Structural Analysis and in Vitro Inhibitory Effect on α-Glucosidase Activity of Millet Bran Dietary Fiber before and after Modification [J]. FOOD SCIENCE, 2018, 39(11): 46-52.
[15]	ZHANG Lu, LIU Pengfei, TU Zongcai, XIE Xing, ZHANG Shuya, SHA Xiaomei, WANG Hui, DAI Huajian. Comparison of Antioxidant Activity and Enzyme Inhibitory Activity of Different Plant Parts of Torreya grandis cv. Merrillii [J]. FOOD SCIENCE, 2018, 39(10): 78-83.

Establishment of Activity Assays for α-Glucosidase and Dipeptidyl Peptidase IV Using Porcine Small Intestine Mucosal Extracts

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments