Abstract: The effect of prodelphinidin extracts from Chinese bayberry leaves at different processing stages (leaf powder, non-desugarized prodelphinidin extract, and desugarized prodelphinidin extract) on pancreatic lipase and phospholipase A1 was evaluated in vitro and in the small intestine of SD rats in comparison with Orlistat. The results indicated that Orlistat had the highest inhibition percentage against pancreatic lipase in vitro and in the small intestine of SD rats, which were 99.0% and 96.5%, respectively, followed by the purified prodelphinidins (90.9% and 91.4%, respectively). Also, Orlistat had the highest inhibition percentage against phospholipase A1 in vitro and in the small intestine of SD rats, which were 97.1% and 97.2%, respectively, followed by the purified prodelphinidins (96.7% and 87.7%, respectively). The half maximal inhibitory concentration of the purified prodelphinidins against pancreatic lipase and phospholipase A1 were 0.01 and 0.06 mg/mL, respectively. The type of inhibition was competitive + uncompetitive for pancreatic lipase and was competitive for phospholipase A1. The purified prodelphinidins from Chinese bayberry leaves are potentially an efficient and controllable anti-obesity agent.

Key words: Chinese bayberry leaves, prodelphinidins, pancreatic lipase, phospholipase A1, anti-obesity