Determination of Dextransucrase-Catalyzed Reaction Products of Sucrose by High Performance Gel Filtration Chromatography with Tandem Two Columns and Analysis of the Dextran Polymerization Process

doi:10.7506/spkx1002-6630-201812030

FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (12): 191-198.doi: 10.7506/spkx1002-6630-201812030

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Determination of Dextransucrase-Catalyzed Reaction Products of Sucrose by High Performance Gel Filtration Chromatography with Tandem Two Columns and Analysis of the Dextran Polymerization Process

HUANG Shuangxia1, HOU Dianzhi1,2, CHEN Hualei1, YU Yue1, CHEN Shan1,3,*

(1. College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China; 2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 3. Collaborative Innovation Center for Guangxi Sugar Industry, Nanning 530004, China)

Online:2018-06-25 Published:2018-06-15

Abstract

Abstract: A precise method for the determination of changes in the molecular mass of dextran and the quantification of sucrose, fructose and glucose concentration during the dextransucrase-catalyzed reaction of sucrose was presented in this paper. High performance gel filtration chromatography (HPGFC) with one column and with two tandem columns were compared for their efficiencies in the determination of dextran standard and the catalytic reaction products in order to develop a robust method to measure the molecular mass of dextran. By dextransucrase combined with two-stage membrane treatment, we investigated the mechanism underlying dextran synthesis. It was found that the synthesis process was continuous aggregation of glucosyl group at the active site of dextransucrase until reaching a specific molecular mass (> 106 Da) before detachment rather than stepwise aggregation and then detachment.

Key words: high performance gel filtration chromatography (HPGFC), two tandem columns, molecular mass of dextran, aggregation process

CLC Number:

TS201.3

HUANG Shuangxia, HOU Dianzhi, CHEN Hualei, YU Yue, CHEN Shan,. Determination of Dextransucrase-Catalyzed Reaction Products of Sucrose by High Performance Gel Filtration Chromatography with Tandem Two Columns and Analysis of the Dextran Polymerization Process[J]. FOOD SCIENCE, 2018, 39(12): 191-198.

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Determination of Dextransucrase-Catalyzed Reaction Products of Sucrose by High Performance Gel Filtration Chromatography with Tandem Two Columns and Analysis of the Dextran Polymerization Process

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