FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (19): 198-203.doi: 10.7506/spkx1002-6630-20190816-179

• Nutrition & Hygiene • Previous Articles     Next Articles

Mulberry Leaf Alkaloids Improve D-Galactose-Induced Oxidative Damage in the Mouse Kidney

YANG Zhongmin, SHEN Yihong, WANG Zuwen, HUANG Xianzhi, DING Xiaowen   

  1. (1. Chongqing Key Laboratory of Agricultural Products Processing, National Demonstration Center for Experimental Food Science and Technology Education, College of Food Science, Southwest University, Chongqing 400716, China; 2. Science and Technology Department, Southwest University, Chongqing 400716, China)
  • Online:2020-10-15 Published:2020-10-23

Abstract: Objective: To explore whether mulberry leaf alkaloids can improve oxidative damage in the kidney of mice for the purpose of providing a theoretical basis for the development and utilization of mulberry leaf alkaloids. Methods: A mouse model of renal oxidative damage was induced by D-galactose (D-Gal) and the model mice were administered with different doses of mulberry leaf alkaloids for 4 consecutive weeks. At the end of the 4th week, the levels of protein carbonyl (PCO), advanced oxidation protein products (AOPP), 8-iso-prostaglandin F2α (8-iso-PGF2α), 8-hydroxy-2’-desoxyguanosine (8-OH-dG) and 5-hydroxy-2’-deoxycotosine (5-OH-dC), and the activity of total superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), reduced nicotinamide adenine dinucleotide phosphate oxidase (NOX), and protein kinase C (PKC) in the kidney was measured. Results: Compared with the model control group, high-dose mulberry leaf alkaloids (200 mg/kg mb) could reduce the protein oxidation products PCO and AOPP in the kidney tissues of mice by 37.27% and 42.71%, respectively (P < 0.01), the lipid oxidation product 8-iso-PGF2α by 51.07% (P < 0.01), and the DNA oxidation products 8-OH-dG and 5-OH-dC by 31.66% and 18.91%, respectively (P < 0.01); increase the activity of antioxidant enzymes SOD and GSH-Px by 83.74% and 59.31%, respectively; and decrease NOX and PKC activity by 41.21% and 40.65%, respectively (P < 0.01). Conclusion: Mulberry leaf alkaloids have a good effect on improving D-Gal-induced oxidative damage in the mouse kidney, and the underlying mechanism may be related to increasing the activity of SOD and GSH-Px and inhibiting the activity of NOX and PKC, thereby reducing the overproduction of reactive oxygen species (ROS) in the body.

Key words: mulberry leaf alkaloids; D-galactose; oxidative damage; kidney damage; mechanism

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