Detection of Aflatoxin B1 in Pixian Broad-Bean Paste by Using Hybridization Chain Reaction Based on Aptamer

doi:10.7506/spkx1002-6630-20190903-037

FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (22): 301-307.doi: 10.7506/spkx1002-6630-20190903-037

• Safety Detection • Previous Articles Next Articles

Detection of Aflatoxin B1 in Pixian Broad-Bean Paste by Using Hybridization Chain Reaction Based on Aptamer

HUANG Yukun, TAO Xuan, SHAO Kun, WANG Chong, WANG Lijun, CHE Zhenming, CHEN Xianggui

(1. School of Food and Biological Engineering, Xihua University, Chengdu 610039, China; 2. Key Laboratory of Food Non Thermal Processing, Engineering Technology Research Center of Food Non Thermal Processing, Yibin Xihua University Research Institute, Yibin 644004, China)

Online:2020-11-25 Published:2020-11-26

Abstract

Abstract: A fluorescence method for the detection of aflatoxin B1 (AFB1) in Pixian broad-bean paste by using hybridization chain reaction (HCR) based on aptamer was established. The effects of the concentration of cDNA, HP1, HP2 and graphene oxide (GO), and incubation time for hybridization were studied on the performed of the method. The optimal conditions were obtained as follows: 50 nmol/L cDNA, 60 nmol/L HP1, 60 nmol/L HP2, 60 min incubation time, and 50 μg/mL GO. Under these conditions, the method showed a good linear relationship in the concentration range of 2–60 ng/mL, the detection limit was 1.84 ng/mL, and the recovery for spiked samples were 85.73%–94.25%. When the method was applied to the detection of 12 different brands of Pixian broad-bean pastes, satisfactory results were obtained which showed no significant difference from those of enzyme-linked immunosorbent assay in the national standard (P > 0.05). This method can detect AFB1 in various Pixian broad-bean pastes easily, quickly and sensitively, and has the potential for fast detection of AFB1 in complex samples.

Key words: aflatoxin B1; aptamer; graphene oxide; hybridization chain reaction; Pixian broad-bean paste

CLC Number:

TS207.3

HUANG Yukun, TAO Xuan, SHAO Kun, WANG Chong, WANG Lijun, CHE Zhenming, CHEN Xianggui. Detection of Aflatoxin B1 in Pixian Broad-Bean Paste by Using Hybridization Chain Reaction Based on Aptamer[J]. FOOD SCIENCE, 2020, 41(22): 301-307.

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Detection of Aflatoxin B1 in Pixian Broad-Bean Paste by Using Hybridization Chain Reaction Based on Aptamer

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