Effect of Thyme Essential Oil Microcapsules on Mechanism of Histamine Production by Morganella morganii and Proteus bacillus

doi:10.7506/spkx1002-6630-20190923-268

Abstract

Abstract: This study explored the effects of thyme essential oil microcapsules on the growth, gene expression and histamine accumulation of Morganella morganii ND and Proteus bacillus R3. High-performance liquid chromatography was used to detect the accumulation of histamine during 48 h culture of the tested strains, and reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used to evaluate the expression of histidine decarboxylase (HDC) pathway-related genes under the influence of thyme essential oil microcapsules. The results showed that thyme essential oil microcapsules had strong antibacterial activity against the test bacteria. The minimal inhibitory concentration (MIC) was 1.56 and 0.78 mg/mL for M. morganii and P. bacillus, respectively. Thyme essential oil microcapsules could reduce histamine accumulation by inhibiting the expression of histidine decarboxylase (hdcA) and histidine/histamine antiporter (hdcP) genes in the HDC cluster. The accumulation of histamine could be reduced by 61.08% and 55.89% in M. morganii and P. bacillus by thyme essential oil microcapsules at MIC concentration.

Key words: thyme essential oil microcapsules; Morganella morganii; Proteus bacillus; histamine; histidine decarboxylase; gene expression

CLC Number:

TS207.7

YU Honghong, WU Mingying, WANG Qingling, DONG Juan, LU Shiling. Effect of Thyme Essential Oil Microcapsules on Mechanism of Histamine Production by Morganella morganii and Proteus bacillus[J]. FOOD SCIENCE, 2020, 41(19): 9-16.

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Effect of Thyme Essential Oil Microcapsules on Mechanism of Histamine Production by Morganella morganii and Proteus bacillus

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