FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (6): 229-237.doi: 10.7506/spkx1002-6630-20191014-106

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Analysis of Differential Composition between Not from Concentrate and from Concentrate Orange Juices Using UPLC-QTOF-MS-Based Metabolomics

LIU Hanlu, ZHANG Jiukai, HAN Jianxun, SUN Ruixue, CHEN Ying, LIU Bing   

  1. (1. Chinese Academy of Inspection and Quarantine, Beijing 100176, China; 2. State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China)
  • Online:2021-03-25 Published:2021-03-29

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Abstract: This study analyzed the differential composition between not from concentrate (NFC) and from concentrate (FC) orange juices using non-targeted metabolomics. Metabolite information from the samples was acquired in both the positive ion and negative ion modes by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). NFC and FC orange juice were distinguished from each other by orthogonal partial least squares discriminant analysis (OPLS-DA), and 16 compounds that significantly differed (P < 0.05) in abundance between them were selected and identified according to the importance in projection values and t-test analysis, including flavonoids, organic acids and alkaloids. The contents of these components in FC orange juice were significantly lower than in NFC orange juice. The results of this study provide theoretical reference for the quality evaluation and identification of NFC juice.

Key words: not from concentrate orange juice; ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry; metabolomics; chemometrics

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