FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (2): 312-318.doi: 10.7506/spkx1002-6630-20200113-129

• Safety Detection • Previous Articles     Next Articles

Rapid Detection of Shiga Toxin-producing Escherichia coli by Immunochromatography Combined with Asymmetric Polymerase Chain Reaction

SHAN Shan, HUANG Zhaohong, HUANG Yanmei, LIU Daofeng, LIU Chengwei, HUANG Yunhong, LONG Zhong’er   

  1. (1. Nanchang Key Laboratory of Microbial Resources Exploitation & Utilization from Poyang Lake Wetland, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China; 2. Jiangxi Yeli Medical Device Co. Ltd., Nanchang 330008, China;3. Jiangxi Province Key Laboratory of Diagnosing and Tracing of Foodborne Disease, Jiangxi Province Center for Disease Control and Prevention, Nanchang 330029, China)
  • Online:2021-01-18 Published:2021-01-27

Abstract: Shiga toxin-producing Escherichia coli (STEC) is strongly pathogenic. Establishing a simple and rapid method for STEC detection is of importance for controlling the occurrence and spread of foodborne outbreaks. In this paper, the biotinylated target single stranded DNA was obtained by asymmetric polymerase chain reaction (aPCR) with the biotinylated downstream primers of the typical virulence genes stx1 and stx2. The DNA amplification products were conjugated with the upstream primers which were labeled on polystyrene microspheres. The biotin from the conjugates could be captured by streptavidin sprayed on a lateral flow strip, forming a red line visible to the naked eye. STEC strains producing different types of Shiga toxin could be detected by this method. It displayed a high specificity and accuracy for detecting 23 strains.

Key words: asymmetric polymerase chain reaction; immunochromatography; Shiga toxin-producing Escherichia coli; detection

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