FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (4): 240-246.doi: 10.7506/spkx1002-6630-20200214-140

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Analysis of Polyphenol Compounds in Different Parts of Ginseng (Panax ginseng C.A. Meyer) Produced in China by HPLC

XU Yanyang, ZHAO Yujuan, GAO Feng, WANG Erlei, LU Hailing, LI Xuefeng, JIANG Wenwen, CHEN Yan   

  1. (1. College of Food Science and Engineering, Jilin University, Changchun 130062, China;2. Hunchun Huarui Shenye Bioengineering Co. Ltd., Hunchun 133000, China)
  • Online:2021-02-25 Published:2021-02-25

Abstract: Objective: To establish a high performance liquid chromatography (HPLC) method for the simultaneous determination of 18 polyphenol compounds (protocatechuic acid, gentisic acid, p-hydroxybenzoic acid, syringic acid, chlorogenic acid p-coumaric acid, ferulic acid, m-coumaric acid, o-coumaric acid, t-cinnamic acid, naringin, catechin, naringenin, formononetin, maltol, hesperetin, vanillin, and resveratrol) in different parts of ginseng produced in China. Methods: The contents of total phenolic and individual compounds in Radix Ginseng rubra (red ginseng), dried ginseng, ginseng stems, leaves, flowers and tassels were determined. The chromatographic separation was achieved on a Symmetry?C18 column (4.6 mm × 150 mm, 5 μm) using a mobile phase consisting of 0.1% phosphoric acid (A) and acetonitrile (B), and the detection wavelength was set as 230 nm. Results: The 18 phenolic compounds were separated within 35 min, with good repeatability (relative standard deviation (RSD) ≤ 3.49%), high precision (RSD ≤ 3.02%), good stability (RSD ≤ 2.58%) and reliable recovery (average recoveries ranging from 84% to 99%, RSD ≤ 5%). Significant differences in the contents of total phenolic and individual compounds were found among different parts of ginseng (P < 0.05), and the contents of maltol and catechin were high in all samples. The contents of total polyphenols in red ginseng, dried ginseng, ginseng stems, leaves, flowers and tassels were (69.47 ± 3.25), (95.04 ± 5.03), (175.19 ± 3.26), (256.91 ± 2.81), (174.40 ± 6.26), and (99.31 ± 2.90) mg/100 g, respectively. Conclusion: The method is simple, efficient, accurate and reliable, and can be used for quality control of polyphenols in ginseng.

Key words: Chinese ginseng; polyphenol compounds; total polyphenols; high performance liquid chromatography

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