Abstract: A high performance anion exchange chromatography with pulsed amperometric detection method was developed for qualitatively and quantitatively analyzed 11 sugars in Laminaria japonica hydrolysate, including fucose, glucose, galactosamine, glucosamine, galactose, mannose, fructose, ribose, lactose, galacturonic acid and glucuronic acid. The separation was carried out on a CarboPac PA10 anion-exchange column (250 mm × 4 mm) through gradient elution using a mobile phase consisting of water, 200 mmol/L NaOH and 500 mmol/L CH3COONa at a flow rate of 1 mL/min. The column temperature was set at 25 ℃. The correlation coefficients (R2) of the calibration curves for all analytes were higher than 0.998 6, and the detection limits (RSN = 3) were in range of 1.27–47.49 mg/kg. The precision expressed as relative standard deviations (RSDs) were smaller than 1.78% and the average spiked recoveries ranged from 89.35% to 115.67%. Therefore, the developed method is simple to operate, and highly linear, sensitive, precise and reproducible.

Key words: high performance anion exchange chromatography; pulsed amperometric detection; Laminaria japonica; monosaccharide