Transcriptomic Analysis of the Mechanism Underlying the Effect of Methyl Jasmonate on the Size of Agaricus bisporus

doi:10.7506/spkx1002-6630-20200720-258

FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (4): 130-137.doi: 10.7506/spkx1002-6630-20200720-258

• Bioengineering • Previous Articles Next Articles

Transcriptomic Analysis of the Mechanism Underlying the Effect of Methyl Jasmonate on the Size of Agaricus bisporus

SONG Yuan, HU Qiuhui, SU Anxiang, PEI Fei, MA Gaoxing, MA Ning, YANG Wenjian

(Key Laboratory of Grains and Oils Quality Control and Processing, Collaborative Innovation Center for Modern Grain Circulation and Safety, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China)

Online:2021-02-25 Published:2021-02-25

Abstract

Abstract: In order to study the effect of methyl jasmonate (MeJA) on the size of Agaricus bisporus, different concentrations of MeJA were sprayed on the mushroom before harvest. The results showed that compared to the control group, 50 μmol/L MeJA significantly increased the average mass of individual mushroom bodies and the diameter and thickness of pilei, and advanced the time to reach the harvest size by one day. The activity of cytochrome P450 oxidase significantly increased on day 1 and day 3 (P < 0.05), and the activity of endo-β-1,4-glucanase significantly increased on day 2 (P < 0.05) compared to the control group. According to the transcriptome sequencing, 525 genes in the pilei of MeJA treated A. bisporus were found to be differentially expressed between day 0 and day 1, significantly more than those found in the control and ethanol treated groups (P < 0.05, |log2Fold Change| > 1). Gene ontology (GO) enrichment analysis showed that MeJA may increase the size of A. bisporus by affecting the differential expression of genes enriched in GO items related to growth and development such as cellular process, cell, and catalytic activity. The gene screening results showed that MeJA could up-regulate the gene AGABI2DRAFT_43781, and down-regulate the genes AGABI2DRAFT_136361, AGABI2DRAFT_188444 and AGABI2DRAFT_194024 to increase the size of A. bisporus. This study provides experimental and theoretical basis that MeJA increases the size of A. bisporus.

Key words: Agaricus bisporus; methyl jasmonate; individual body size; transcriptome sequencing; differentially expressed genes

CLC Number:

TS255.3

SONG Yuan, HU Qiuhui, SU Anxiang, PEI Fei, MA Gaoxing, MA Ning, YANG Wenjian. Transcriptomic Analysis of the Mechanism Underlying the Effect of Methyl Jasmonate on the Size of Agaricus bisporus[J]. FOOD SCIENCE, 2021, 42(4): 130-137.

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Transcriptomic Analysis of the Mechanism Underlying the Effect of Methyl Jasmonate on the Size of Agaricus bisporus

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