Abstract: A new rapid method for the simultaneous qualitative and quantitative analysis of aspartame and alitame in beverage and yogurt was established using reversed-phase high performance liquid chromatography with mixed standard and sample increment method. The results showed that the minimum detection limits of aspartame and alitame in beverages and liquid dairy products were 0.21 and 0.020 mg/kg, respectively. The recoveries ranged from 90.5% to 98.3%, with relative standard deviation (RSD) less than 5%. The results obtained from the proposed method showed no significant difference compared with the national standard method. This method does not need to measure blanks and draw a standard curve. It is simple, fast and low-cost, and allows the simultaneous qualitative and quantitative analysis of standard and sample solutions under the same conditions. Satisfactory results can be obtained when it is used for the rapid determination of aspartame and alitame in food.

Key words: reversed-phase high performance liquid chromatography; mixed standard solution; incremental sampling method; aspartame; alitame; simultaneous qualitative and quantitative analysis