Abstract: In this study, based on the CaMV35S promoter, the NOS terminator, Cry1Ab/Ac, HPT and the rice endogenous gene SPS, five different fluorescent probes (FAM, HEX, Taxas Red, Cy5, and Cy5.5) were used to develop and optimize a pentaplex real-time polymerase chain reaction (real-time PCR) method to detect genetically modified rice. For this purpose, a series of experiments was carried out to screen primer combinations, optimize the reaction system, and evaluate the specificity, sensitivity and applicability. The sensitivity of the proposed method was 0.032%. This method has the characteristics of high sensitivity, accuracy and high throughput and allows the rapid and efficient detection of genetically modified components in rice.

Key words: genetically modified rice; screening element; single real-time polymerase chain reaction; pentaplex real-time polymerase chain reaction; high-throughput detection method