Abstract: This study was performed in order to explore the regulatory mechanism of nitrogen catabolite repression (NCR) in Debaryomyces hansenii cultured under two different nitrogen levels: with (experimental group) and without (control group) L-histidine addition. The Illumina high-throughput sequencing platform was used for transcriptome sequencing of both groups, and various bioinformatics methods were used for data analysis and processing. A total of 42.921 6 and 41.415 4 M clean reads were obtained for the control and experimental groups. Furthermore, a total of 133 significantly differentially expressed genes were identified between the two groups, 78 of which were up-regulated, while 50 were down-regulated. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that most of the differentially expressed genes were involved in histamine metabolism-related coenzymes. This study provides a reference for further studies on histamine metabolism in D. hansenii.

Key words: Debaryomyces hansenii; transcriptome; nitrogen catabolite repression; histamine