FOOD SCIENCE ›› 2022, Vol. 43 ›› Issue (6): 81-88.doi: 10.7506/spkx1002-6630-20210220-219
• Bioengineering • Previous Articles Next Articles
ZHANG Yu, XIA Li, LIN Beibei, ZHANG Wenjie, XU Haoran, ZHANG Chenglin
Online:
Published:
Abstract: Nutrients such as L-threonine need to be added during the fermentation of L-homoserine by auxotrophic strains. In this study, a non-auxotrophic Escherichia coli chassis for the high-yield production of L-homoserine was constructed. The expression of thrB and thrC were down-regulated to attenuate the degradation of L-homoserine; then the overexpression of thrA, ppc and pntAB was performed to reinforce the synthetic flux and improve precursor supply and NADPH generation; finally, rhtA was overexpressed to elevate L-homoserine efflux. After 48 h fermentation in a 5 L fermenter, the constructed strain H-10 produced 52.1 g/L of L-homoserine with a percentage transformation of glucose of 43%, which is higher than the values reported in the literature.
Key words: L-homoserine; metabolic engineering; Escherichia coli; efflux
CLC Number:
Q939.97
ZHANG Yu, XIA Li, LIN Beibei, ZHANG Wenjie, XU Haoran, ZHANG Chenglin. Construction of Escherichia coli Chassis for Efficient Production of L-Homoserine[J]. FOOD SCIENCE, 2022, 43(6): 81-88.
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URL: https://www.spkx.net.cn/EN/10.7506/spkx1002-6630-20210220-219
https://www.spkx.net.cn/EN/Y2022/V43/I6/81