Effect of Browning Reaction on the Metabolite Profile of Yogurt Analyzed by Untargeted Metabolomics Based on Ultra-high Performance Liquid Chromatography-Quadrupole Electrostatic Field Orbitrap Mass Spectrometry

doi:10.7506/spkx1002-6630-20210504-019

FOOD SCIENCE ›› 2022, Vol. 43 ›› Issue (6): 272-278.doi: 10.7506/spkx1002-6630-20210504-019

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Effect of Browning Reaction on the Metabolite Profile of Yogurt Analyzed by Untargeted Metabolomics Based on Ultra-high Performance Liquid Chromatography-Quadrupole Electrostatic Field Orbitrap Mass Spectrometry

LI Meng, NING Xuenan, KANG Jiaxin, LIAO Minhe, REN Haowei, LIU Ning

(Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China)

Online:2022-03-25 Published:2022-03-28

Abstract

Abstract: In this study, untargeted metabolomics based on ultra-high performance liquid chromatography-quadrupole electrostatic field orbitrap mass spectrometry (UPLC-QE-MS) was used to explore the differences in metabolite profiles between ordinary yogurt and brown yogurt, and the differences between them were evaluated from the perspective of metabolites. The metabolite information of yogurts was collected in both the positive and negative ion modes, and the metabolites were screened by multivariate statistical analysis. A total of 117 differential metabolites were obtained. Compared with ordinary yogurt, the relative contents of 106 metabolites were up-regulated and 11 metabolites were down-regulated in brown yogurt. Compared with ordinary yogurt, the contents of 3 flavor amino acids, 8 peptides, 12 sugars, 2 Maillard reaction products and one quinone in brown yogurt changed significantly. The results of this study can provide some theoretical support for the future research and development of brown yogurt.

Key words: brown yogurt; browning reaction; metabonomics; differential metabolites

CLC Number:

TS252.54

LI Meng, NING Xuenan, KANG Jiaxin, LIAO Minhe, REN Haowei, LIU Ning. Effect of Browning Reaction on the Metabolite Profile of Yogurt Analyzed by Untargeted Metabolomics Based on Ultra-high Performance Liquid Chromatography-Quadrupole Electrostatic Field Orbitrap Mass Spectrometry[J]. FOOD SCIENCE, 2022, 43(6): 272-278.

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Effect of Browning Reaction on the Metabolite Profile of Yogurt Analyzed by Untargeted Metabolomics Based on Ultra-high Performance Liquid Chromatography-Quadrupole Electrostatic Field Orbitrap Mass Spectrometry

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