A Fluorescent Aptasensor Based on Fe3+ Doped Polydopamine Nanospheres for Detection of Deoxynivalenol in Wheat

doi:10.7506/spkx1002-6630-20220228-241

FOOD SCIENCE ›› 2023, Vol. 44 ›› Issue (4): 313-320.doi: 10.7506/spkx1002-6630-20220228-241

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A Fluorescent Aptasensor Based on Fe3+ Doped Polydopamine Nanospheres for Detection of Deoxynivalenol in Wheat

LU Nian, MA Ji,, CHENG Junhu, SUN Da-wen

(1. School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; 2. Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou 510006, China; 3. Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou 510006, China; 4. State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, China)

Published:2023-03-01

Abstract

Abstract: Objective: A fluorescent aptasensor based on fluorescence resonance energy transfer (FRET) was constructed for the detection of deoxynivalenol (DON) in wheat. The ability of polydopamine nanospheres (PDANS) in the sensor to quench 6-carboxyuorescein (FAM) was improved by doping with Fe3+. Methods: FAM labeled DON aptamer (PDON) was adsorbed on the surface of PDANS (Fe-PDANS) doped with Fe3+ via π-π stacking non-covalent interactions, and Fe-PDANS quenched the fluorescence of FAM through FRET. In the presence of DON, PDON could specifically combine with DON, changing the conformation of PDON, making it difficult to adsorb onto Fe-PDANS, and finally enhancing the fluorescence signal of the sensor for the detection of DON. Results: The aptasensor exhibited a linear range from 0.266 7 to 133.3 ng/mL for DON, and the detection limit was 0.118 2 ng/mL. The results of this aptasensor for DON in positive wheat samples were not significantly different (P > 0.05) from those obtained by enzyme-linked immunosorbent assay (ELISA). Conclusion: The fluorescent aptasensor is characterized by rapid detection, excellent sensitivity, high specificity and good repeatability, which will provide a new method for the detection of DON in wheat.

Key words: aptamer; deoxynivalenol; Fe3+ doped polydopamine nanospheres; fluorescent sensor

CLC Number:

TS207

LU Nian, MA Ji,, CHENG Junhu, SUN Da-wen. A Fluorescent Aptasensor Based on Fe3+ Doped Polydopamine Nanospheres for Detection of Deoxynivalenol in Wheat[J]. FOOD SCIENCE, 2023, 44(4): 313-320.

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A Fluorescent Aptasensor Based on Fe3+ Doped Polydopamine Nanospheres for Detection of Deoxynivalenol in Wheat

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