Untargeted Metabolomics Based on Ultra-high Performance Liquid Chromatography-Mass Spectrometry for Analysis of Differential Metabolites in Lactobacillus-fermented Ultra-fine Whole Pulp of Chimonobambusa quadrangularis Shoot

doi:10.7506/spkx1002-6630-20221019-177

Abstract

Abstract: In this work, a non-targeted metabolomic study was performed on the ultrafine whole pulp of Lactobacillus-fermented Chimonobambusa quadrangularis shoot using ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS). Based on the variable importance in projection (VIP) > 1 and P < 0.05 in t-test, 361, 392 and 398 differential metabolites were selected in raw (control) versus Limosilactobacillus reuteri (LR) fermented samples, control versus Lactobacillus plantarum (LP) fermented samples, and control and LR + LP (LRP) fermented samples in the positive ion mode; and 268, 304 and 321 differential metabolites in the negative ion mode, respectively. The major differential metabolites were lipids and lipid-like compounds, organic acids and their derivatives, organic heterocyclic compounds, and benzenes including histamine, D-mannan, indole-3-lactic acid, DL-4-hydroxyphenyl lactate, ornithine, and proline. Four significant metabolic pathways were identified by Kyoto Encyclopedia of Genes and Genomes metabolic pathway enrichment analysis, namely purine metabolism, pyrimidine metabolism and phenylalanine metabolism and citric acid cycle. This study provides theoretical support for the development of fermented bamboo shoot products.

Key words: Chimonobambusa quadrangularis shoot; untargeted metabolomics; differential metabolites; Limosilactobacillus reuteri; Lactobacillus plantarum

CLC Number:

TS255.5

Wang Lei, Jia Yulong, Luo Yanyu, ZOU Chunxia, ZHANG Ying. Untargeted Metabolomics Based on Ultra-high Performance Liquid Chromatography-Mass Spectrometry for Analysis of Differential Metabolites in Lactobacillus-fermented Ultra-fine Whole Pulp of Chimonobambusa quadrangularis Shoot[J]. FOOD SCIENCE, 2023, 44(18): 200-213.

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[2]	LUO Xuan, CHENG Cheng, ZHANG Qi, WANG Yunpu, SONG Xiaoxiao, XIE Honglei, LIU Yuhuan, CUI Xian. Effects of Physicochemical Properties and Microbial Communities of Fermented Soybean Products on the Formation of Biogenic Amines [J]. FOOD SCIENCE, 2023, 44(14): 72-78.
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[4]	CHEN Tianyan, SU Wei, MU Yingchun, YIN Xuedong. Correlation Analysis between Microorganisms and Volatile Flavor Substances during the Post-ripening of Fermented Bamboo Shoots with Chopped Pepper [J]. FOOD SCIENCE, 2023, 44(6): 180-189.
[5]	Ye-Xin TIAN. Correlation between microorganisms and volatile flavor compounds in the post ripening stage of chopped pepper and ginger shreds [J]. FOOD SCIENCE, 0, (): 0-0.
[6]	. Mechanism and Influencing Factors on Color Formation of Fermented Vegetables [J]. FOOD SCIENCE, 0, (): 0-0.
[7]	YANG Shan, WANG Wei, ZHAO Nan, HUANG Fude, ZENG Xueqing, LAI Haimei, MEI Yuan, ZHU Yongqing. Recent Advances in Understanding Mechanism and Influential Factors of Color Formation in Fermented Vegetables [J]. FOOD SCIENCE, 2022, 43(23): 269-276.
[8]	. Analysis of Differential Metabolites and Related Metabolism Pathways of Different Long-term Fermented Kohlrabi [J]. , 0, (): 0-0.
[9]	NIE Qianli, WANG Xiujun, LIU Linxin, CHEN Yanhong, BAO Huanhuan, HE Chunxia. Analysis of Bacterial Flora Structure and Flavor Quality of Bacterial-type Naturally Fermented Dried Douchi Produced in Zunyi [J]. FOOD SCIENCE, 2022, 43(14): 158-164.
[10]	Qian-Li NIE Huanhuan BAO Chun-Xia HE. Analysis on the Flora Structure and Flavor Quality of Zunyi Natural Fermented Dried Bacterial-type Douchi [J]. FOOD SCIENCE, 0, (): 0-0.
[11]	Tian-Yan CHEN. Effects of microorganisms on volatile flavor compounds during post ripening of chopped pepper mountain bamboo shoots [J]. FOOD SCIENCE, 0, (): 0-0.
[12]	JIan Ming. Effects of Curing Treatment Combined with Different Drying Methods on the Quality Characteristics and Microstructure of Chestnut Flours [J]. FOOD SCIENCE, 0, (): 0-0.
[13]	XUE Ailian, XIA Xiaoxia, KOU Fubing, RAN Huan, LEI Xiaojuan, ZHAO Jichun, ZENG Kaifang, MING Jian. Effect of Cooking Combined with Different Drying Methods on the Quality Characteristics and Microstructure of Chinese Chestnut Powder [J]. FOOD SCIENCE, 2022, 43(9): 62-70.
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[15]	子叶 Jun-Jie YI. Untargeted analysis on flavor differences of different fermented chili peppers （Xiaomila） based on the HS-SPME-GC-MS [J]. FOOD SCIENCE, 0, (): 0-0.

Untargeted Metabolomics Based on Ultra-high Performance Liquid Chromatography-Mass Spectrometry for Analysis of Differential Metabolites in Lactobacillus-fermented Ultra-fine Whole Pulp of Chimonobambusa quadrangularis Shoot

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