Abstract: The inhibitory effect of fumigation with 1-octen-3-ol against the growth and deoxynivalenol (DON) biosynthesis of Fusarium graminearum PH-1 was determined, and the possible mechanism involved was investigated by evaluating cell membrane integrity, oxidative stress level and the expression of key genes related to DON biosynthesis. The results showed that 1-octen-3-ol significantly inhibited the mycelial growth, spore germination and mycotoxin production of F. graminearum in a concentration-dependent manner (P < 0.05). After 7 days of fumigation with 100 μL/L 1-octen-3-ol, the inhibition rates of mycelial growth, spore germination and deoxynivalenol production were 60.70%, 100.00% and 97.50%, respectively. Further studies showed that 1-octen-3-ol treatment effectively damaged the cell membrane integrity and increased the membrane permeability of F. graminearum, leading to a significant reduction of ergosterol levels and serious leakage of cell contents. Moreover, 1-octen-3-ol interfered with the oxidative stress balance of F. graminearum and downregulated the expression of DON biosynthesis-related genes such as TRI4, TRI5, TRI8, TRI10, TRI12, and TRI101. In conclusion, 1-octen-3-ol can effectively inhibit the growth and DON biosynthesis of F. graminearum by damaging cell membrane integrity, interfering with oxidative stress balance and regulating key gene expression.

Key words: 1-octen-3-ol; Fusarium graminearum; deoxynivalenol; inhibitory mechanism